WebThin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. [1] Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or … WebOne of those methods is known as thin-layer chromatography, or TLC for short. This works like all other kinds of chromatography in that you have a mobile phase and a stationary phase, and the compounds that you're trying to separate interact with these two phases. First, let's talk about the stationary phase.
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